InnoSpin Blood DNA Extraction Kit

The InnoSpin Blood DNA Extraction Kit purifies high-quality genomic DNA from whole blood and buffy coat samples using silica membrane spin column technology. A fast, reagent-safe workflow delivers concentrated, PCR-ready DNA for routine molecular diagnostic applications.

FORMAT

Spin Column

PREPS

50 Reactions

SAMPLE VOLUME

200 µL

Pack Size (Reactions): 25 Reactions (MBDS-01 25T)

25 Reactions (MBDS-01 25T) 50 Reactions (MBDS-01L) 100 Reactions (MBDS-01C)

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QUICK HIGHLIGHTS

Silica Column Technology

High Yield and Purity

Fast Extraction Workflow

PCR, qPCR and NGS Ready

Room Temperature Storage

No Phenol or Chloroform

KIT OVERVIEW

Engineered for Precision DNA Extraction

The InnoSpin Blood DNA Extraction Kit uses silica membrane spin-column technology to selectively bind, wash, and elute high-quality genomic DNA from whole blood and buffy coat samples. The chaotropic salt-based chemistry ensures complete cell disruption while optimised binding conditions maximise DNA capture from every sample.

Compatible with EDTA, citrate, and heparin-treated blood, the kit fits into standard collection workflows without requiring additional pre-treatment steps. The spin-column format provides a reliable and reproducible workflow that integrates easily into routine laboratory operations with a streamlined, room-temperature workflow.

Technology

Silica Spin-Column Membrane

Sample Type

Whole Blood, Buffy Coat

Sample Volume

200 µL

DNA Yield (Whole Blood)

5 to 9 µg per 200 µL

DNA Yield (Buffy Coat)

8 to 12 µg per 200 µL

DOWNSTREAM APPLICATIONS

PCR and Real-Time PCR (qPCR)

Purified DNA provides a suitable starting material for PCR and real-time PCR workflows that require reliable nucleic acid quality. The extracted DNA can support target amplification and detection in compatible molecular testing applications, including diagnostic and research workflows.

DOWNSTREAM APPLICATIONS

SNP Genotyping

SNP genotyping workflows require DNA of suitable quality and quantity for the detection and analysis of genetic variations. DNA extracted using the InnoSpin Blood DNA Extraction Kit can support compatible genotyping methods, providing purified starting material for downstream analysis of specific genetic markers.

DOWNSTREAM APPLICATIONS

Next-Generation Sequencing (NGS)

Next-generation sequencing requires nucleic acid that meets the quality and quantity requirements of the intended sequencing workflow. Purified DNA from the InnoSpin Blood DNA Extraction Kit can support compatible NGS applications, including downstream library preparation and genomic analysis.

DOWNSTREAM APPLICATIONS

Microarray Analysis

Microarray applications use DNA to investigate genetic markers, variations, and other molecular characteristics. The purified DNA obtained through the InnoSpin Blood DNA Extraction Kit can support compatible microarray-based analysis where the extracted DNA meets the requirements of the specific platform and protocol.

DOWNSTREAM APPLICATIONS

Restriction Enzyme Digestion

Restriction enzyme digestion uses specific enzymes to cleave DNA at defined recognition sites and is commonly used in molecular biology workflows. Purified DNA from the InnoMag extraction process can provide suitable starting material for compatible enzymatic digestion and subsequent molecular analysis.

THE PROCESS

Simple Steps Pure Results

HOW IT WORKS

Step 1 – Lysis

Add whole blood or buffy coat to the lysis buffer. Incubate to fully disrupt cells and release genomic DNA under chaotropic conditions.

HOW IT WORKS

Step 2 – Binding

Transfer the lysate to the silica membrane spin column. Centrifuge briefly to bind DNA selectively to the membrane.

HOW IT WORKS

Step 3 – Wash

Wash the column with two sequential wash buffers to remove proteins, salts, and other contaminants.

HOW IT WORKS

Step 4 – Elution

Elute purified DNA with a low volume of elution buffer. Collect purified DNA suitable for downstream molecular applications.

Supporting callouts (below steps)

Streamlined extraction workflow
No phenol or chloroform
Room temperature throughout

Quick Answers
Here

Find quick answers about the InnoSpin Blood DNA Extraction Kit, including protocol guidance, sample compatibility, yield expectations, and ordering.

Get More Details

Which blood collection tube types are compatible?

The InnoSpin Blood DNA Extraction Kit is designed for DNA extraction from whole blood and other compatible blood-derived sample types. For recommended sample collection and handling guidelines, please refer to the product instructions or contact our technical team.

What DNA yield can I expect from whole blood?

DNA yield may vary depending on the sample type, quality, and processing conditions. The kit is designed to deliver high-quality genomic DNA suitable for a wide range of downstream molecular applications when used according to the recommended protocol.

How long does the protocol take?

The extraction workflow is designed to be simple and efficient, helping laboratories process samples with minimal hands-on time. Please refer to the product instructions for the complete protocol and recommended processing steps.

Does the kit require any hazardous reagents?

No. The InnoSpin Blood DNA Extraction Kit is designed to provide a straightforward DNA extraction workflow without the use of phenol or chloroform, making routine laboratory handling safer and more convenient.

Does the protocol require refrigeration?

The kit is designed for convenient laboratory use, with components stored according to the recommended storage conditions provided in the product instructions. Please follow the storage guidelines to ensure optimal performance.

What downstream applications is the extracted DNA suitable for?

The purified DNA is suitable for a variety of downstream molecular applications, including PCR, qPCR, next-generation sequencing (NGS), genotyping, restriction enzyme digestion, and other molecular biology workflows that require high-quality genomic DNA.

Can I request a sample for evaluation?

Yes. Evaluation samples may be available for qualifying institutional buyers and diagnostic laboratories. Share your application details and product requirements through the enquiry form, and our team will review your request before getting in touch with the next steps. Our team can also help you identify the most suitable solution for your laboratory's requirements.

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Where Accuracy Meets Accessibility

Diagnostic solutions built for laboratories, hospitals, and healthcare institutions, designed for real-world testing needs.

Contact

sales@medinno.in

InnoSpin Blood DNA Extraction Kit

Engineered for Precision DNA Extraction

PCR and Real-Time PCR (qPCR)

SNP Genotyping

Next-Generation Sequencing (NGS)

Microarray Analysis

Restriction Enzyme Digestion

Step 1 – Lysis

Step 2 – Binding

Step 3 – Wash

Step 4 – Elution

Quick Answers
Here