InnoMag Sputum DNA Extraction Kit

The InnoMag Sputum DNA Extraction Kit isolates Mycobacterium tuberculosis DNA from human sputum specimens using paramagnetic bead technology. Built for viscous and mucous-heavy samples, it delivers high-yield, inhibitor-free DNA ready for qPCR, digital PCR, and NGS.

FORMAT

Magnetic Bead

PREPS

50 Reactions

SAMPLE VOLUME

500 µL to 1 mL

Pack Size (Reactions): 25 Reactions (MBDM-02 25T)

QUICK HIGHLIGHTS

Magnetic Bead Technology
High-Efficiency MTB Lysis
No Centrifugation Required
Inhibitor-Free DNA
Automation Compatible
qPCR, dPCR & NGS Ready

KIT OVERVIEW

Reliable DNA Extraction from the Most Challenging Sputum Samples

The InnoMag Sputum DNA Extraction Kit combines optimised lysis chemistry with magnetic bead purification to address the specific challenges of sputum samples. A tailored buffer system and Proteinase K break down the lipid-rich cell wall of Mycobacterium tuberculosis, while paramagnetic beads capture DNA selectively, even from samples with low bacterial loads.

The magnetic separation process removes heme, polysaccharides, and other PCR inhibitors commonly found in sputum, without the need for centrifugation or organic solvents. The kit delivers clean, PCR-ready DNA suitable for high-throughput workflows and compatible with automated liquid handling platforms.

Technology

Paramagnetic Bead Separation

Sample Type

Human Sputum

Sample Volume

500 µL to 1 mL

Target Organism

Mycobacterium tuberculosis (MTB)

Purity (A260/A280)

1.7 to 2.0

WHY INNOMAG SPUTUM

Magnetic Bead Technology 

Efficient magnetic bead-based DNA isolation for consistent recovery.

WHY INNOMAG SPUTUM

High DNA Purity 

Produces DNA suitable for PCR, sequencing, and genotyping applications.

WHY INNOMAG SPUTUM

Simple Workflow 

No centrifugation or organic solvents required.

WHY INNOMAG SPUTUM

Optimised Sample Volume 

Designed to process 500 µL to 1 mL of human sputum samples for efficient DNA extraction.

WHY INNOMAG SPUTUM

Room Temperature Storage 

Supports convenient storage and transport of dried blood spot samples.

THE PROCESS

Simple Steps Pure Results

HOW IT WORKS

Step 1 - Lysis

Add the sputum sample to the tailored lysis buffer with Proteinase K. Incubate to fully disrupt the MTB cell wall and release genomic DNA.

HOW IT WORKS

Step 2 - Binding

Add paramagnetic beads and binding buffer. Mix to allow selective DNA capture onto the bead surface.

HOW IT WORKS

Step 3 - Wash

Apply magnetic separation. Sequential wash steps remove heme, polysaccharides, and PCR inhibitors while preserving DNA quality.

HOW IT WORKS

Step 4 - Elution

Add elution buffer. Collect purified, inhibitor-free DNA ready for immediate downstream use.

Supporting callouts (below steps)

  • No centrifugation required
  • No organic solvents
  • Complete in 40 minutes

Quick Answers
Here

Find quick answers about the InnoMag Sputum DNA Extraction Kit, including protocol guidance, sample requirements, compatibility, and ordering.

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